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All aspects of SAXS from macromolecules in solution

Small angle X-ray scattering (SAXS) is a powerful method to analyse proteins and other macromolecules in solution and can give tremendous insights for example on the effects of buffer composition, the quaternary state of proteins as well as their interactions with other macromolecules or small ligands.

SAXS elucidates:

• overall particle shape;
• quaternary structure;
• conformational changes (e.g. on ligand binding);
• complex formation;
• oligomerization behaviour;
• influence of buffer conditions/temperature;
• assembly and folding;
• flexibility of fragments or entire structures;
• interparticle interactions;
• particle size distributions.

Required: about 0.5 mg of purified material at concentration ~0.5-5 mg/ml.

And don't forget the matching buffers!

How it works

Describe the problem

• Discuss your project with experts

Send the samples

• SAXS measurements at the world's most brilliant synchrotron
• Data analysis and model building

Receive the answers

• Summary of results including interpretation of the SAXS models
• Within weeks time and under non-disclosure agreement

Options: mail-in operation, remote data collection, personal visit. Service within weeks time under non-disclosure agreement.

Send your target molecules and buffer(s) according to their required stability conditions (e.g. cooled or on dry ice). Your samples will be stored exactly according to your instructions upon arrival.

We work with monodisperse or moderately polydisperse solutions of

• proteins,
• RNA, DNA,
• macromolecular complexes,
• nanoparticles,
• colloids,
• gels.