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All aspects of SAXS from macromolecules in solution

Small angle X-ray scattering (SAXS) is a powerful method to analyse proteins and other macromolecules in solution and can give tremendous insights for example on the effects of buffer composition, the quaternary state of proteins as well as their interactions with other macromolecules or small ligands.

SAXS elucidates:

  • overall particle shape;
  • quaternary structure;
  • conformational changes (e.g. on ligand binding);
  • complex formation;
  • oligomerization behaviour;
  • influence of buffer conditions/temperature;
  • assembly and folding;
  • flexibility of fragments or entire structures;
  • interparticle interactions;
  • particle size distributions.
SAXS data analysis
SAXS samples

Required: about 0.5 mg of purified material at concentration ~0.5-5 mg/ml.

And don't forget the matching buffers!

How it works

Describe the problem

  • Discuss your project with experts

Send the samples

  • SAXS measurements at the world's most brilliant synchrotron
  • Data analysis and model building

Receive the answers

  • Summary of results including interpretation of the SAXS models
  • Within weeks time and under non-disclosure agreement

Options: mail-in operation, remote data collection, personal visit. Service within weeks time under non-disclosure agreement.

Send your target molecules and buffer(s) according to their required stability conditions (e.g. cooled or on dry ice). Your samples will be stored exactly according to your instructions upon arrival.

We work with monodisperse or moderately polydisperse solutions of

  • proteins,
  • RNA, DNA,
  • macromolecular complexes,
  • nanoparticles,
  • colloids,
  • gels.

Training

The BIOSAXS team not only has many years of hands-on experience in this technology but also a strong background in training of candidates from pharma and biotech companies as well as academic groups. According to your needs we offer customized training courses covering all aspects of SAXS experiments:

  • experiment design, sample preparation, small angle scattering theory, data analysis, modelling using the ATSAS package;
  • one-to-one training or a group course with up to 20 participants;
  • in our training rooms in Hamburg or for your convenience at your own site.

Please, contact us and let us know what type of training would be beneficial for your needs.

SAXS in the Pharmaceutical Industry, June 2021